Journal: Advanced healthcare materials

Article Title: Hydrogel microtumor arrays to evaluate nanotherapeutics

doi: 10.1002/adhm.202201696

Figure Lengend Snippet: Mechanical cues prime a population of cells with increase stemness which increase uptake of Cy5-PFC3 (A) Representative image of fluorescence intensity quantitation in B16F0 cells (Image J). Cells outlined in red considered ‘perimeter’ and yellow considered ‘centre’. Heatmap of nanoparticle intensity after five days culture (n=10). (B) Scatter plot of fluorescence intensity of B16F0 cells localised at the perimeter and the centre of a pattern cultured for five days after 1 h treatment with Cy5-PFC3. Significant difference in uptake between cells in perimeter and centre (p = 0.0002, n = 12) and ABCB5 expression (p = 0.0079, n = 12). (C) Scatter plot of fluorescence intensity of B16F0 and B16F10 cells in spiral patterns cultured on 10 kPa polyacrylamide substrate. Error bars denote ± standard deviation. Significant difference in Cy5-PFC3 uptake between B16F0 1 day and 5 days (p = 0.0005, n = 15), B16F0 and B16F10 at 5 days (p < 0.0001, n = 15) and ABCB5 expression between B16F0 1 day and 5 day (p < 0.0001, n = 15), B16F0 and B16F10 at 5 days (p < 0.0174, n = 20)

Article Snippet: Gels were then incubated with the primary antibody (rabbit polyclonal anti-ABCB5, Novus Biologicals) diluted at 1:500 in 1% BSA in PBS for 1h at room temperature.

Techniques: Fluorescence, Quantitation Assay, Cell Culture, Expressing, Standard Deviation

Journal: Advanced healthcare materials

Article Title: Hydrogel microtumor arrays to evaluate nanotherapeutics

doi: 10.1002/adhm.202201696

Figure Lengend Snippet: Shape induced and innate cellular ABCB5 expression increases nanoparticle uptake in murine melanoma cells. (Scale bar = 100 μm) (A) Representative fluorescence images of a B16F0 one day culture, B16F0 five day culture, B16F10 one day culture, and B16F10 five day culture. Fluorescence channels present Cy5-PFC3 nanoparticle (Red), ABCB5 putative cancer stem cell marker (Alexa Fluor 555, Orange) and cancer cell nuclei (DAPI) on spiral patterns. (B) Quantification of mean Cy5-PFC3 and ABCB5 stem cell marker intensity in B16F0 (n = 10) and B16F10 (n = 10) cells proliferated under spiral geometry for one day or five days with standard deviation shown with error bars. ****: P ≤ 0.0001, ***: P ≤0.001

Article Snippet: Gels were then incubated with the primary antibody (rabbit polyclonal anti-ABCB5, Novus Biologicals) diluted at 1:500 in 1% BSA in PBS for 1h at room temperature.

Techniques: Expressing, Fluorescence, Marker, Standard Deviation

Journal: Cancer Drug Resistance

Article Title: The uniqueness of ABCB5 as a full transporter ABCB5FL and a half-transporter-like ABCB5β

doi: 10.20517/cdr.2024.56

Figure Lengend Snippet: Cancers in which ABCB5 expression has been identified outside of its role as a marker of CSC

Article Snippet: Immunohistochemistry using antibody NBP1-77687 from Novus Biological , [ ] .

Techniques: Expressing, Marker, Next-Generation Sequencing, Sequencing, Immunohistochemistry, DNA Sequencing